Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

被引：28

作者：

McConnell, G

Riis, E

机构：

[1] Univ Strathclyde, Ctr Biophoton, SIBS, Glasgow G4 0NR, Lanark, Scotland

[2] Univ Strathclyde, Dept Phys, Glasgow G4 0NG, Lanark, Scotland

来源：

PHYSICS IN MEDICINE AND BIOLOGY | 2004年 / 49卷 / 20期

关键词：

D O I：

10.1088/0031-9155/49/20/007

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.

引用

收藏

页码：4757 / 4763

页数：7

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