Thermodynamic properties of the bovine serum albumin-sodium taurodeoxycholate system

被引：11

作者：

Antonelli, ML ^{[1
]}

Capalbi, A ^{[1
]}

Gente, G ^{[1
]}

Palacios, AC ^{[1
]}

Sallustio, S ^{[1
]}

La Mesa, C ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2004年 / 246卷 / 1-3期

关键词：

proteins; BSA; bile salts; NaTDC; surface properties; colligative properties; solution calorimetry; thermodynamics; interactions;

D O I：

10.1016/j.colsurfa.2004.02.032

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The interaction between bovine serum albumin, BSA, and sodium taurodeoxycholate, NaTDC, a component of the bile in mammals, has been investigated for several protein to surfactant ratios. The solution region was investigated by surface tension, sigma, freezing point depression, DeltaT, emf measurements, as well as by integral heat of dilution data, DeltaH(i,dil). The corresponding properties of NaTDC in aqueous solutions were investigated too. Surfactant binding onto the protein is controlled by a delicate balance of hydrophobic and electrostatic contributions, responsible for the adduct stability, but does not give rise to precipitation or coacervate formation, at least at pH around 6.0. The observed behaviour is in line with current knowledge on the solution behaviour of BSA-TDC complexes occurring in hepatic and gallbladder bile. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：127 / 134

页数：8

共 44 条

[1] Interactions between water-soluble polymers and surfactants: Effect of the polymer hydrophobicity .2. Amphiphilic polyelectrolytes (polysoaps) [J].

Anthony, O ;

Zana, R .

LANGMUIR, 1996, 12 (15) :3590-3597

[2] SOLUTION PROPERTIES OF OCTYL-BETA-D-GLUCOSIDE .2. THERMODYNAMICS OF MICELLE FORMATION [J].

ANTONELLI, ML ;

BONICELLI, MG ;

CECCARONI, G ;

LAMESA, C ;

SESTA, B .

COLLOID AND POLYMER SCIENCE, 1994, 272 (06) :704-711

[3] DIELECTRIC-PROPERTIES OF THE SYSTEM BOVINE ALBUMIN-UREA-BETAINE IN AQUEOUS-SOLUTION [J].

BATEMAN, JB ;

EVANS, GF ;

BROWN, PR ;

GABRIEL, C ;

GRANT, EH .

PHYSICS IN MEDICINE AND BIOLOGY, 1992, 37 (01) :175-182

[4] STATIC AND DYNAMIC STUDIES OF SODIUM TAURODEOXYCHOLATE MICELLAR AGGREGATION IN WATER IN THE RANGE 25-45-DEGREES-C [J].

BERCHIESI, G ;

BERCHIESI, MA ;

LAMESA, C ;

SESTA, B .

JOURNAL OF PHYSICAL CHEMISTRY, 1984, 88 (16) :3665-3669

[5] Structural study of the micellar aggregates of sodium taurodeoxycholate [J].

Bonincontro, A ;

Briganti, G ;

DArchivio, AA ;

Galantini, L ;

Giglio, E .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (49) :10303-10309

[6] On the composition of sodium taurodeoxycholate micellar solutions [J].

Bottari, E ;

Festa, MR .

LANGMUIR, 1996, 12 (07) :1777-1783

[7] Aggregation behavior of bile salts in aqueous solution [J].

Coello, A ;

Meijide, F ;

Nunez, ER ;

Tato, JV .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1996, 85 (01) :9-15

[8] Polymer-surfactant interactions: An ultrasonic relaxation study [J].

DAprano, A ;

LaMesa, C ;

Persi, L .

LANGMUIR, 1997, 13 (22) :5876-5880

[9] THERMODYNAMIC PROPERTIES OF ALKYLDIMETHYLAMINE OXIDES IN WATER - APPLICATION OF A MASS-ACTION MODEL FOR MICELLIZATION [J].

DESNOYERS, JE ;

CARON, G ;

DELISI, R ;

ROBERTS, D ;

ROUX, A ;

PERRON, G .

JOURNAL OF PHYSICAL CHEMISTRY, 1983, 87 (08) :1397-1406

[10] Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: spectroscopy and modelling [J].

Gelamo, EL ;

Silva, CHTP ;

Imasato, H ;

Tabak, M .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1594 (01) :84-99

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