DIELECTRIC-PROPERTIES OF THE SYSTEM BOVINE ALBUMIN-UREA-BETAINE IN AQUEOUS-SOLUTION

被引：18

作者：

BATEMAN, JB ^{[1
]}

EVANS, GF ^{[1
]}

BROWN, PR ^{[1
]}

GABRIEL, C ^{[1
]}

GRANT, EH ^{[1
]}

机构：

[1] UNIV LONDON KINGS COLL,DEPT BIOCHEM,LONDON WC2R 2LS,ENGLAND

来源：

PHYSICS IN MEDICINE AND BIOLOGY | 1992年 / 37卷 / 01期

关键词：

D O I：

10.1088/0031-9155/37/1/012

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Urea (about 5 mol l-1) causes an approximately five-fold increase of the dielectric increment of bovine serum albumin at 20-degrees-C. The increase is reversed by betaine (about 2.5 mol l-1), or prevented if urea and betaine are added together. This result can be seen as an electric counterpart to the protective role of osmolytes which are secreted by cells and tissues subjected to various types of internal or environmental stress.

引用

页码：175 / 182

页数：8

共 43 条

[1] PREFERENTIAL INTERACTIONS OF PROTEINS WITH SOLVENT COMPONENTS IN AQUEOUS AMINO-ACID SOLUTIONS [J].

ARAKAWA, T ;

TIMASHEFF, SN .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 224 (01) :169-177

[2] PROTEIN STABILIZATION AND DESTABILIZATION BY GUANIDINIUM SALTS [J].

ARAKAWA, T ;

TIMASHEFF, SN .

BIOCHEMISTRY, 1984, 23 (25) :5924-5929

[3] THE STABILIZATION OF PROTEINS BY OSMOLYTES [J].

ARAKAWA, T ;

TIMASHEFF, SN .

BIOPHYSICAL JOURNAL, 1985, 47 (03) :411-414

[4] INDUCTION OF ALDOSE REDUCTASE AND SORBITOL IN RENAL INNER MEDULLARY CELLS BY ELEVATED EXTRACELLULAR NACL [J].

BAGNASCO, SM ;

UCHIDA, S ;

BALABAN, RS ;

KADOR, PF ;

BURG, MB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (06) :1718-1720

[5] PARTIAL SPECIFIC VOLUMES IN HIGHLY CONCENTRATED PROTEIN SOLUTIONS .1. WATER-BOVINE SERUM-ALBUMIN AND WATER-BOVINE HEMOGLOBIN [J].

BERNHARDT, J ;

PAULY, H .

JOURNAL OF PHYSICAL CHEMISTRY, 1975, 79 (06) :584-590

[6]

BRESLER SE, 1949, BIOKHIMIYA, V14, P180

[7]

CHEN RF, 1967, J BIOL CHEM, V242, P173

[8]

Cohn E. J., 1943, PROTEINS AMINO ACIDS

[9] PREPARATION AND PROPERTIES OF SERUM AND PLASMA PROTEINS .13. CRYSTALLIZATION OF SERUM ALBUMINS FROM ETHANOL WATER MIXTURES [J].

COHN, EJ ;

HUGHES, WL ;

WEARE, JH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1947, 69 (07) :1753-&

[10] THE HOFMEISTER EFFECT AND THE BEHAVIOR OF WATER AT INTERFACES [J].

COLLINS, KD ;

WASHABAUGH, MW .

QUARTERLY REVIEWS OF BIOPHYSICS, 1985, 18 (04) :323-422

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