The rat brain hippocampus proteome

被引：50

作者：

Fountoulakis, M ^{[1
]}

Tsangaris, G

Maris, A

Lubec, G

机构：

[1] F Hoffmann La Roche Ltd, Ctr Med Genom, Basel, Switzerland

[2] Acad Athens, Fdn Biomed, Ctr Basic Res, Athens 11527, Greece

[3] Univ Vienna, Fac Med, Dept Pediat, Vienna, Austria

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 819卷 / 01期

关键词：

rat brain; hippocampus; proteome; proteomics; two-dimensional protein database; mass spectrometry; memory;

D O I：

10.1016/j.jchromb.2005.01.037

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The hippocampus is crucial in memory storage and retrieval and plays an important role in stress response. In humans, the CA1 area of hippocampus is one of the first brain areas to display pathology in Alzheimer's disease. A comprehensive analysis of the hippocampus proteome has not been accomplished yet. We applied proteomics technologies to construct a two-dimensional database for rat brain hippocampus proteins. Hippocampus samples from eight months old animals were analyzed by two-dimensional electrophoresis and the proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The database comprises 148 different gene products, which are in the majority enzymes, structural proteins and heat shock proteins. It also includes 39 neuron specific gene products. The database may be useful in animal model studies of neurological disorders. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：115 / 129

页数：15

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