Application of ultrasonic technique for extracting chlorogenic acid from Eucommia ulmodies Oliv. (E ulmodies)

被引：158

作者：

Li, H

Chen, B

Yao, SZ ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biol Sensing & Chemometr, Changsha 410082, Peoples R China

[2] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Peoples R China

来源：

ULTRASONICS SONOCHEMISTRY | 2005年 / 12卷 / 04期

基金：

中国国家自然科学基金;

关键词：

ultrasound; chlorogenic acid; Euconnnia uhnodies oliv; extraction;

D O I：

10.1016/j.ultsonch.2004.01.033

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

An ultrasonic method for the extraction of chlorogenic acid from fresh leaves of Eucominia ulmodies Oliv. was investigated and optimized. The influence of four extraction variables on extraction efficiency of chlorogenic acid was investigated. The optimum extraction conditions found were: 70% aqueous methanol; solvent: sample ratio = 20:1 (v/w); extraction time 3 x 30 min. The recovery of chlorogenic acid was studied (HPLC) and the reproducibility of the extraction method was determined. The optimized ultrasonic extraction conditions were applied to extract chlorogenic acid from fresh leaves, fresh bark and dried bark of E. ulmodies and four traditional Chinese medicines. The application of sonication method was shown to be highly efficient in the extraction of chlorogenic acid from E. ulmodies and other Chinese medicines compared with classical methods. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：295 / 300

页数：6

共 24 条

[1]

DEYAMA T, 1987, CHEM PHARM BULL, V35, P1785

[2] Comparison of ultrasound-assisted extraction and microwave-assisted digestion for determination of magnesium, manganese and zinc in plant samples by flame atomic absorption spectrometry [J].