Expansion microscopy

被引：225

作者：

Cho, I. ^{[1
]}

Seo, J. Y. ^{[1
]}

Chang, J. ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Dept Biomed Engn, Suwon, South Korea

来源：

JOURNAL OF MICROSCOPY | 2018年 / 271卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Brain imaging; Expansion microscopy; Super-resolution microscopy; FLUORESCENT PROTEINS; ORGANIZATION; ANTIBODIES; TISSUES; REVEALS;

D O I：

10.1111/jmi.12712

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Super-resolution optical microscopy techniques have revolutionized how we see and understand biology. In recent past, a new super-resolution optical microscopy technique called expansion microscopy (ExM) was developed. Unlike other pre-existing super-resolution imaging techniques, this technique achieves super-resolution by physically expanding biological specimens via a swellable hydrogel. After the development of ExM, various techniques based on ExM but with improved performance in various aspects, have been developed. In this review, we introduce the basic principles of ExM and its variants. and compare the advantages and disadvantages of these techniques. In addition, we present the applications of ExM techniques in various fields.

引用

页码：123 / 128

页数：6

共 11 条

[1] Superresolution expansion microscopy reveals the three-dimensional organization of the Drosophila synaptonemal complex [J].

Cahoon, Cori K. ;

Yu, Zulin ;

Wang, Yongfu ;

Guo, Fengli ;

Unruh, Jay R. ;

Slaughter, Brian D. ;

Hawley, R. Scott .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (33) :E6857-E6866

[2]

Chang JB, 2017, NAT METHODS, V14, P593, DOI [10.1038/NMETH.4261, 10.1038/nmeth.4261]

[3] Nanoscale imaging of RNA with expansion microscopy [J].

Chen, Fei ;

Wassie, Asmamaw T. ;

Cote, Allison J. ;

Sinha, Anubhav ;

Alon, Shahar ;

Asano, Shoh ;

Daugharthy, Evan R. ;

Chang, Jae-Byum ;

Marblestone, Adam ;

Church, George M. ;

Raj, Arjun ;

Boyden, Edward S. .

NATURE METHODS, 2016, 13 (08) :679-+

[4] Expansion microscopy [J].

Chen, Fei ;

Tillberg, Paul W. ;

Boyden, Edward S. .

SCIENCE, 2015, 347 (6221) :543-548

[5]

Chozinski TJ, 2016, NAT METHODS, V13, P485, DOI [10.1038/NMETH.3833, 10.1038/nmeth.3833]

[6] Expansion microscopy of zebrafish for neuroscience and developmental biology studies [J].

Freifeld, Limor ;

Odstrcil, Iris ;

Foerster, Dominique ;

Ramirez, Alyson ;

Gagnon, James A. ;

Randlett, Owen ;

Costa, Emma K. ;

Asano, Shoh ;

Celiker, Orhan T. ;

Gao, Ruixuan ;

Martin-Alarcon, Daniel A. ;

Reginato, Paul ;

Dick, Cortni ;

Chen, Linlin ;

Schoppik, David ;

Engert, Florian ;

Baier, Herwig ;

Boyden, Edward S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (50) :E10799-E10808

[7] Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization [J].

Halpern, Aaron R. ;

Alas, Germain C. M. ;

Chozinski, Tyler J. ;

Paredez, Alexander R. ;

Vaughan, Joshua C. .

ACS NANO, 2017, 11 (12) :12677-12686

[8] Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues [J].

Ku, Taeyun ;

Swaney, Justin ;

Park, Jeong-Yoon ;

Albanese, Alexandre ;

Murray, Evan ;

Cho, Jae Hun ;

Park, Young-Gyun ;

Mangena, Vamsi ;

Chen, Jiapei ;

Chung, Kwanghun .

NATURE BIOTECHNOLOGY, 2016, 34 (09) :973-+

[9] Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies [J].

Tillberg, Paul W. ;

Chen, Fei ;

Piatkevich, Kiryl D. ;

Zhao, Yongxin ;

Yu, Chih-Chieh ;

English, Brian P. ;

Gao, Linyi ;

Martorell, Anthony ;

Suk, Ho-Jun ;

Yoshida, Fumiaki ;

DeGennaro, Ellen M. ;

Roossien, Douglas H. ;

Gong, Guanyu ;

Seneviratne, Uthpala ;

Tannenbaum, Steven R. ;

Desimone, Robert ;

Cai, Dawen ;

Boyden, Edward S. .

NATURE BIOTECHNOLOGY, 2016, 34 (09) :987-+

[10] Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications [J].

Zhang, Yu Shrike ;

Chang, Jae-Byum ;

Alvarez, Mario Moises ;

Trujillo-de Santiago, Grissel ;

Aleman, Julio ;

Batzaya, Byambaa ;

Krishnadoss, Vaishali ;

Ramanujam, Aishwarya Aravamudhan ;

Kazemzadeh-Narbat, Mehdi ;

Chen, Fei ;

Tillberg, Paul W. ;

Dokmeci, Mehmet Remzi ;

Boyden, Edward S. ;

Khademhosseini, Ali .

SCIENTIFIC REPORTS, 2016, 6

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