Omeprazole induces apoptosis in Jurkat cells

被引：37

作者：

Scaringi, L ^{[1
]}

Cornacchione, P

Ayroldi, E

Corazzi, L

Capodicasa, E

Rossi, R

Marconi, P

机构：

[1] Univ Perugia, Gen Hosp, Dept Clin & Expt Med, Gen Pathol & Immunol Sect, I-06122 Perugia, Italy

[2] Univ Perugia, Gen Hosp, Pharmacol Toxicol & Chemotherapy Sect, I-06122 Perugia, Italy

[3] Univ Perugia, Gen Hosp, Dept Internal Med, Biochem Sect, I-06122 Perugia, Italy

[4] Univ Perugia, Gen Hosp, Internal Med & Oncol Sci Sect, I-06122 Perugia, Italy

来源：

INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY | 2004年 / 17卷 / 03期

关键词：

omeprazole; apoptosis; lysosome; Jurkat T cell; cathepsins; caspases;

D O I：

10.1177/039463200401700313

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We report for the first time a potent apoptotic effect of omeprazole (OM). Apoptosis was induced in Jurkat cells in a time and concentration-dependent mode. Caspase 3 and PARP were rapidly cleaved in response to OM, but apoptosis was only partially inhibited by the caspase 3 inhibitor DEVD-CHO. OM also induced an early lysosomal destabilization which increased progressively and was correlated with a parallel increase in apoptotic cells. The cysteine protease inhibitor E64d gave strong protection against apoptosis thus proving the involvement of lysosomal enzymes in OM-induced apoptosis whereas, it did not impede the caspase 3 cleavage. Instead ZVAD-fmk, a general caspase inhibitor, also able to inhibit cathepsin activity, protected cells completely from OM-induced apoptosis. It therefore seems that both caspases and cysteine cathepsins are involved in the execution stage of OM-induced apoptosis.

引用

页码：331 / 342

页数：12

共 64 条

[1] H2O2-induced apoptosis of thymocytes involves mobilization of divalent cations [J].