Identification of the clam species Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), and Ruditapes philippinarum (Japanese carpet shell) by PCR-RFLP

被引：27

作者：

Fernández, A ^{[1
]}

García, T ^{[1
]}

Asensio, L ^{[1
]}

Rodríguez, MA ^{[1
]}

González, I ^{[1
]}

Céspedes, A ^{[1
]}

Hernández, PE ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bromatol 3, E-28040 Madrid, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 08期

关键词：

species identification; PCR-RFLP; alpha-actin gene; clam species; Ruditapes decussatus; Venerupis pullastra; Ruditapes philippinarum;

D O I：

10.1021/jf0002185

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

PCR-RFLP analysis has been applied to the identification of three clam species: Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), and Ruditapes philippinarum (Japanese carpet shell). PCR amplification was carried out using a set of primers designed from the DNA nucleotide sequences reported for a-actins from humans and various animals. Restriction endonuclease analysis based on sequence data of the PCR products of each clam species revealed the presence of species-specific polymorphic sites for MaeIII and RsaI endonucleases. Electrophoretic analysis of the amplicons digested with MaeIII and RsaI produced species-specific profiles that allowed the: genetic identification of the three clam species.

引用

页码：3336 / 3341

页数：6

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