Production of reactive oxygen species during non-specific elicitation, non-host resistance and field resistance expression in cultured tobacco cells

被引:35
作者
Able, AJ
Sutherland, MW
Guest, DI
机构
[1] Univ So Queensland, Fac Sci, Ctr Rural & Environm Biotechnol, Toowoomba, Qld 4350, Australia
[2] Univ So Queensland, Fac Sci, Dept Biol & Phys Sci, Toowoomba, Qld 4350, Australia
[3] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia
关键词
disease resistance; glucan elicitors; plant-pathogen interactions; reactive oxygen species;
D O I
10.1071/FP02123
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We examined production of reactive oxygen species (ROS) and induction of cell death in tissue-cultured tobacco cells undergoing different disease resistance responses. A superoxide-dependent hypersensitive response occurs during both the race-specific resistance response of tobacco cells challenged with incompatible zoospores of Phytophthora nicotianae and during non-specific elicitation of tobacco cells challenged with Phytophthora glucan elicitors extracted from the fungal cell wall. Inhibition studies are consistent with dependence upon endogenous Ca2+ levels, and with involvement of NAD(P)H oxidase and peroxidases in production of ROS during both specific and non-specific elicitation. The patterns of resistance expression during non-host resistance or field resistance responses appear to be similar to race-specific resistance expression with regard to the timing and order of events. However, the intensity of the response is very much reduced. In contrast, during non-specific elicitation, these temporal patterns are significantly altered. The differences in timing, intensity and extent of responses during different modes of disease resistance expression indicate that stimulation of cultured plant cells with non-specific soluble fractions in order to model in planta events during plant/Oomycete and, by implication, plant/fungal interactions, has significant limitations.
引用
收藏
页码:91 / 99
页数:9
相关论文
共 52 条
[1]   Relationship between transmembrane ion movements, production of reactive oxygen species and the hypersensitive response during the challenge of tobacco suspension cells by zoospores of Phytophthora nicotianae [J].
Able, AJ ;
Guest, DI ;
Sutherland, MW .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2001, 58 (05) :189-198
[2]   Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of Phytophthora parasitica var nicotianae [J].
Able, AJ ;
Guest, DI ;
Sutherland, MW .
PLANT PHYSIOLOGY, 1998, 117 (02) :491-499
[3]   Hydrogen peroxide yields during the incompatible interaction of tobacco suspension cells inoculated with Phytophthora nicotianae [J].
Able, AJ ;
Guest, DI ;
Sutherland, MW .
PLANT PHYSIOLOGY, 2000, 124 (02) :899-910
[4]   TIMING OF MOLECULAR EVENTS FOLLOWING ELICITOR TREATMENT OF PLANT-CELLS [J].
ANDERSON, AJ ;
ROGERS, K ;
TEPPER, CS ;
BLEE, K ;
CARDON, J .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1991, 38 (01) :1-13
[5]   INVOLVEMENT OF PLASMA-MEMBRANE CALCIUM INFLUX IN BACTERIAL INDUCTION OF THE K+/H+ AND HYPERSENSITIVE RESPONSES IN TOBACCO [J].
ATKINSON, MM ;
KEPPLER, LD ;
ORLANDI, EW ;
BAKER, CJ ;
MISCHKE, CF .
PLANT PHYSIOLOGY, 1990, 92 (01) :215-221
[6]   ROLE OF THE PLASMALEMMA H+-ATPASE IN PSEUDOMONAS-SYRINGAE-INDUCED K+/H+ EXCHANGE IN SUSPENSION-CULTURED TOBACCO CELLS [J].
ATKINSON, MM ;
BAKER, CJ .
PLANT PHYSIOLOGY, 1989, 91 (01) :298-303
[7]   HOST-PATHOGEN INTERACTIONS .9. QUANTITATIVE ASSAYS OF ELICITOR ACTIVITY AND CHARACTERIZATION OF ELICITOR PRESENT IN EXTRACELLULAR MEDIUM OF CULTURES OF PHYTOPHTHORA-MEGA-SPERMA VAR SOJAE [J].
AYERS, AR ;
EBEL, J ;
FINELLI, F ;
BERGER, N ;
ALBERSHEIM, P .
PLANT PHYSIOLOGY, 1976, 57 (05) :751-759
[8]   HOST-PATHOGEN INTERACTIONS .10. FRACTIONATION AND BIOLOGICAL-ACTIVITY OF AN ELICITOR ISOLATED FROM MYCELIAL WALLS OF PHYTOPHTHORA-MEGASPERMA VAR SOJAE [J].
AYERS, AR ;
EBEL, J ;
VALENT, B ;
ALBERSHEIM, P .
PLANT PHYSIOLOGY, 1976, 57 (05) :760-765
[9]   HOST-PATHOGEN INTERACTIONS .11. COMPOSITION AND STRUCTURE OF WALL-RELEASED ELICITOR FRACTIONS [J].
AYERS, AR ;
VALENT, B ;
EBEL, J ;
ALBERSHEIM, P .
PLANT PHYSIOLOGY, 1976, 57 (05) :766-774
[10]  
Baillieul Fabienne, 1996, Molecular Plant-Microbe Interactions, V9, P215