Characterisation of shake flasks for cultivation of animal cell cultures

被引：6

作者：

Jaenicke, G. ^{[1
]}

Sauter, C. ^{[1
]}

Bux, R. ^{[1
]}

Haas, J. ^{[1
]}

机构：

[1] Boehringer Ingelheim Pharma GmbH & Co KG, Birkendorfer Str 65, D-88397 Biberach, Germany

来源：

CELL TECHNOLOGY FOR CELL PRODUCTS | 2007年

关键词：

shake flask; mass transfer; gas-liquid; oxygen; power input; oxygen transfer rate; sulphite system; cell culture; CHO cells; RAMOS; PreSens; dissolved oxygen; mammalian cells; bioreactor; mass transfer coefficient;

D O I：

10.1007/978-1-4020-5476-1_131

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study investigated the oxygen transfer processes and general correlations between culture performance and the operating conditions for shake flask fermentations of animal cell cultures. This involved both the online measurement of the oxygen transfer rate and the continuous recording of measurements for the dissolved oxygen concentration in the shake flask.

引用

页码：727 / +

页数：2