Metabolomics analysis II. Preparation of biological samples prior to detection

被引：112

作者：

Alvarez-Sanchez, B.

Priego-Capote, F. ^{[1
]}

Luque de Castro, M. D.

机构：

[1] Univ Cordoba, Dept Analyt Chem, E-14071 Cordoba, Spain

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2010年 / 29卷 / 02期

关键词：

Clean up; Derivatization; Extraction; Fractionation; Metabolite; Metabolomics; Preconcentration; Quenching; Sample preparation; Separation; 2-DIMENSIONAL GAS-CHROMATOGRAPHY; GLOBAL METABOLITE ANALYSIS; MASS-SPECTROMETRY; INTRACELLULAR METABOLITES; HS-SPME; EXTRACTION; NMR; YEAST; TISSUE; GC;

D O I：

10.1016/j.trac.2009.12.004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

After discussing the significance of preliminary operations, such as selection or storage of biological samples, we critically review the other steps of the analytical process prior to metabolite detection. First comes interruption of metabolism or quenching, which is of crucial importance in the analysis of biological samples. Then, we consider quantitative extraction of metabolites (selective or total for targeted or global metabolomics analysis, respectively) as a function of sample characteristics: solid or liquid samples and cell culture. Finally, we comment on additional steps, such as preconcentration of metabolites, clean up of extracts and fractionation for metabolite separation, and discuss their inclusion in analytical methods. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：120 / 127

页数：8

共 60 条

[1] Highly-parallel metabolomics approaches using LC-MS2 for pharmaceutical and environmental analysis [J].