Ordered cloned DNA map of the genome of Vibrio cholerae 569B and localization of genetic markers

被引：15

作者：

Chatterjee, S ^{[1
]}

Mondal, AK ^{[1
]}

Begum, NA ^{[1
]}

Roychoudhury, S ^{[1
]}

Das, J ^{[1
]}

机构：

[1] Indian Inst Chem Biol, Div Biophys, Calcutta 700032, W Bengal, India

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 04期

关键词：

D O I：

10.1128/JB.180.4.901-908.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol. 176:1105-1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic strain 569B of Vibrio cholerae has been constructed, A cosmid library the size of about 4,000 clones containing more than 120 Mb of V. cholerae genomic DNA (40-genome equivalent) was generated. By combining landmark analysis and chromosome walking, the cosmid clones were assembled into 13 contigs covering about 90% of the V. cholerae genome. A total of 92 cosmid clones were assigned to the genome and to regions defined by NotI, SfiI, and CeuI macrorestriction maps, Twenty-seven cloned genes, 9 rrn operons, and 10 copies of a repetitive DNA sequence (IS1004) have been positioned on the ordered cloned DNA map.

引用

页码：901 / 908

页数：8

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