Micro-scale purification of β-galactosidase from Kluyveromyces lactis reveals that dimeric and tetrameric forms are active

被引：41

作者：

Becerra, M ^{[1
]}

Cerdan, E ^{[1
]}

Siso, MIG ^{[1
]}

机构：

[1] Fac Ciencias, Dept Biol Celular & Mol, A Coruna 15071, Spain

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 03期

关键词：

D O I：

10.1023/A:1008885827560

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-Galactosidase was purified from Kluyveromyces lactis. Polyclonal antibodies raised against it cross-reacted against the beta-galactosidase of Kluyveromyces fragilis but not against beta-galactosidases of bacterial, fungal or animal origin. The enzyme is composed of 124 kDa monomers and is active in both dimeric and tetrameric form.

引用

页码：253 / 256

页数：4

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