TRAMPR: an R package for analysis and matching of terminal-restriction fragment length polymorphism (TRFLP) profiles

被引:41
作者
Fitzjohn, Richard G. [1 ]
Dickie, Ian A. [1 ]
机构
[1] Landcare Res, Lincoln 7640, New Zealand
来源
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 04期
关键词
community analysis; pattern matching; R; TRFLP;
D O I
10.1111/j.1471-8286.2007.01744.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
TRAMPR ( TRFLP analysis and matching package for R) is a package for matching multiple terminal restriction fragment length polymorphism ( TRFLP) profiles between unknown samples and a database of known TRFLP profiles in order to infer the presence of species in environmental samples. It permits simultaneous analysis of multiple samples and facilitates direct workflow from electrophoresis output through to community analyses. TRAMPR also resolves the issues of multiple TRFLP profiles within a species and ( conversely) shared TRFLP profiles across species.
引用
收藏
页码:583 / 587
页数:5
相关论文
共 17 条
[1]   Statistical methods for characterizing diversity of microbial communities by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes [J].
Abdo, Z ;
Schüette, UME ;
Bent, SJ ;
Williams, CJ ;
Forney, LJ ;
Joyce, P .
ENVIRONMENTAL MICROBIOLOGY, 2006, 8 (05) :929-938
[2]   Wood-inhabiting fungal communities in woody debris of Norway spruce (Picea abies(L.) Karst.), as reflected by sporocarps, mycelial isolations and T-RFLP identification [J].
Allmér, J ;
Vasiliauskas, R ;
Ihrmark, K ;
Stenlid, J ;
Dahlberg, A .
FEMS MICROBIOLOGY ECOLOGY, 2006, 55 (01) :57-67
[3]  
[Anonymous], 2006, R LANG ENV STAT COMP
[4]   A 'dirty' business: testing the limitations of terminal restriction fragment length polymorphism (TRFLP) analysis of soil fungi [J].
Avis, PG ;
Dickie, IA ;
Mueller, GM .
MOLECULAR ECOLOGY, 2006, 15 (03) :873-882
[5]   A method to size DNA fragments from 50 to 800 bp on a DNA analyser [J].
Avis, PG ;
Feldheim, KA .
MOLECULAR ECOLOGY NOTES, 2005, 5 (04) :969-970
[6]   Ectomycorrhizal fungi identification in single and pooled root samples: terminal restriction fragment length polymorphism (TRFLP) and morphotyping compared [J].
Burke, DJ ;
Martin, KJ ;
Rygiewicz, PT ;
Topa, MA .
SOIL BIOLOGY & BIOCHEMISTRY, 2005, 37 (09) :1683-1694
[7]   Vertical niche differentiation of ectomycorrhizal hyphae in soil as shown by T-RFLP analysis [J].
Dickie, IA ;
Xu, B ;
Koide, RT .
NEW PHYTOLOGIST, 2002, 156 (03) :527-535
[8]   Inter- and intraspecific resolution of nrDNA TRFLP assessed by computer-simulated restriction analysis of a diverse collection of ectomycorrhizal fungi [J].
Edwards, IP ;
Turco, RF .
MYCOLOGICAL RESEARCH, 2005, 109 :212-226
[9]   Nitrogen availability alters macrofungal basidiomycete community structure in optimally fertilized loblolly pine forests [J].
Edwards, IP ;
Cripliver, JL ;
Gillespie, AR ;
Johnsen, KH ;
Scholler, M ;
Turco, RF .
NEW PHYTOLOGIST, 2004, 162 (03) :755-770
[10]   Fine-scale distribution of pine ectomycorrhizas and their extramatrical mycelium [J].
Genney, DR ;
Anderson, IC ;
Alexander, IJ .
NEW PHYTOLOGIST, 2006, 170 (02) :381-390