Earthworm Extracts Facilitate PC12 Cell Differentiation and Promote Axonal Sprouting in Peripheral Nerve Injury

被引：38

作者：

Chen, Chao-Tsung ^{[1
]}

Lin, Jaung-Geng ^{[1
]}

Lu, Tung-Wu ^{[3
]}

Tsai, Fuu-Jen ^{[1
]}

Huang, Chih-Yang ^{[1
]}

Yao, Chun-Hsu ^{[2
]}

Chen, Yueh-Sheng ^{[1
,2
]}

机构：

[1] China Med Univ, Grad Inst Chinese Med Sci, Taichung 404, Taiwan

[2] China Med Univ, Dept Biomed Imaging & Radiol Sci, Taichung 404, Taiwan

[3] Natl Taiwan Univ, Inst Biomed Engn, Taipei 10764, Taiwan

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2010年 / 38卷 / 03期

关键词：

Earthworm; Peripheral Nerve Regeneration; PC12; Cell; Silicone Rubber; Traditional Chinese Medicine; IN-VITRO; GROWTH-FACTOR; SCIATIC-NERVE; SYNAPSIN-I; REGENERATION; NGF; RATS; COLLAGEN; VIVO;

D O I：

10.1142/S0192415X10008044

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

The present study provides in vitro and in vivo evaluations of earthworm (Pheretima aspergilum) on peripheral nerve regeneration. In the in vitro study, we found the earthworm (EW) water extracts caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from PC12 cells as well as the expressions of growth associated protein 43 and synapsin I. In the in vivo study, silicone rubber chambers filled with EW extracts were used to bridge a 10mm sciatic nerve defect in rats. Eight weeks after implantation, the group receiving EW extracts had a much higher success percentage of regeneration (90%) compared to the control (60%) receiving the saline. In addition, quantitative histology of the successfully regenerated nerves revealed that myelinated axons in EW group at 31.25 mu g/ml was significantly more than those in the controls (p < 0: 05). These results showed that EW extracts can be a potential growth-promoting factor on regenerating peripheral nerves.

引用

页码：547 / 560

页数：14

共 22 条

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Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties [J].