Detection of Gov system antibodies by MAIPA reveals an immunogenicity similar to the HPA-5 alloantigens

被引:74
作者
Berry, JE
Murphy, CM
Smith, GA
Ranasinghe, E
Finberg, R
Walton, J
Brown, J
Navarrete, C
Metcalfe, P
Ouwehand, WH
机构
[1] Univ Cambridge, Dept Haematol, Div Transfus Med, Natl Blood Serv E Anglia, Cambridge CB2 2PT, England
[2] Univ Cambridge, Dept Haematol, Cambridge, England
[3] Natl Blood Serv, London, England
[4] Dana Farber Canc Inst, Boston, MA 02115 USA
[5] Harvard Univ, Sch Med, Boston, MA USA
[6] Natl Inst Biol Stand & Controls, Div Haematol, Potters Bar EN6 3QG, Herts, England
关键词
platelets; monoclonal antibody-specific immobilization; of platelet antigens (MAIPA); Gov system; immunogenicity;
D O I
10.1046/j.1365-2141.2000.02170.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The glycosylphosphatidylinositol-linked platelet protein CD109 carries the biallelic alloantigen system Gov. There is limited information on the incidence of Gov alloantibodies in neonatal alloimmune thrombocytopenia (NAITP), post-transfusion purpura (PTP) and platelet refractoriness. We adapted the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay to the detection of Gov antibodies and determined their incidence in 605 archived samples (112 with HPA antibodies) referred for the aforementioned conditions. Here, we show that CD109 expression was reduced upon platelet storage in saline or by cryopreservation, but was stable when stored as whole blood or therapeutic platelet concentrate. Fourteen of the 605 samples contained Gov alloantibodies (anti-Gov(a), n = 10; anti-Gov(b), n = 4), with the majority in platelet refractoriness (n = 9) and, of the remaining five, four in NAITP and one in PTP In seven cases, no other HPA antibodies were detected, three being NAITP cases. The incidence of Gov antibodies was significantly lower than HPA-1 system antibodies (n = 87), but equalled the number of HPA-5 system antibodies (n = 14) and outnumbered HPA-2 and -3 system antibodies (10 altogether).
引用
收藏
页码:735 / 742
页数:8
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