Oxidation Changes Physical Properties of Phospholipid Bilayers: Fluorescence Spectroscopy and Molecular Simulations

被引：114

作者：

Beranova, Lenka ^{[1
]}

Cwiklik, Lukasz ^{[1
,2
,3
]}

Jurkiewicz, Piotr ^{[1
]}

Hof, Martin ^{[1
]}

Jungwirth, Pavel ^{[2
,3
]}

机构：

[1] Acad Sci Czech Republ, J Heyrovsky Inst Phys Chem, VVI, CR-18223 Prague 8, Czech Republic

[2] Acad Sci Czech Republ, Inst Organ Chem & Biochem, CR-16610 Prague 6, Czech Republic

[3] Ctr Biomol & Complex Mol Syst, Prague 16610 6, Czech Republic

来源：

LANGMUIR | 2010年 / 26卷 / 09期

关键词：

OXIDIZED PHOSPHOLIPIDS; LIPID-PEROXIDATION; IN-VIVO; DYNAMICS; RECEPTOR; STRESS; HYDRATION; BINDING;

D O I：

10.1021/la100657a

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Physical properties of oxidized phospholipid (OxPL) membranes consisting of binary mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 10 mol% of one of two OxPLs, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) or 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), were investigated experimentally and computationally. Fluorescence solvent relaxation (SR) and fluorescence correlation spectroscopy z-scan (FCS z-scan) show increased headgroup hydration and mobility, and faster lateral diffusion in POPC membrane upon addition of OxPLs. The magnitudes of both effects arc distinct for each of the two OxPLs. Molecular dynamics simulations corroborate the experimental findings, providing at the same time a detailed molecular interpretation in terms of changes in bilayer structure and phospholipid orientation.

引用

页码：6140 / 6144

页数：5

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