Subsecond chiral separations on a microchip

被引：63

作者：

Piehl, N ^{[1
]}

Ludwig, M ^{[1
]}

Belder, D ^{[1
]}

机构：

[1] Max Planck Inst Kohlenforsch, Abteilung Chromatog, D-45470 Mulheim, Germany

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 21-22期

关键词：

amino acids; chiral separation; cyclodextrin; high-throughput screening; microchip electrophoresis; microfluidics; miniaturization;

D O I：

10.1002/elps.200406028

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fast chiral separation of DNS-amino acids could be realized using microchip electrophoresis with fluorescence detection. For this purpose, highly sulfated cyclodextrins (HS-gamma-CD) were used as chiral selectors enabling high selectivity. Even subsecond separation of DNS-tryptophan, DNS-norleucine, DNS-phenylalanine, DNS-methionine, and DNS-aspartic acid could be achieved. Baseline separation could be accomplished within 720 ms, which is the fastest separation of enantiomers reported to date. A more complex mixture consisting of three chiral DNS-amino acids could be separated within 3.3 s utilizing a separation length of only 7 mm and an electrical field strength of 2012 V/cm.

引用

页码：3848 / 3852

页数：5

共 48 条

[1]

Amini A, 2001, ELECTROPHORESIS, V22, P3107, DOI 10.1002/1522-2683(200109)22:15<3107::AID-ELPS3107>3.0.CO

[2]

2-Z

[3] Surface modification in microchip electrophoresis [J].

Belder, D ;

Ludwig, M .

ELECTROPHORESIS, 2003, 24 (21) :3595-3606

[4] Microchip electrophoresis for chiral separations [J].

Belder, D ;

Ludwig, M .

ELECTROPHORESIS, 2003, 24 (15) :2422-2430

[5]

Belder D, 2002, ELECTROPHORESIS, V23, P2355, DOI 10.1002/1522-2683(200207)23:14<2355::AID-ELPS2355>3.0.CO

[6]

2-Q

[7] Very fast enantioseparation in high-performance liquid chromatography using cellulose tris(3,5-dimethylphenylcarbamate) coated on monolithic silica support [J].

Chankvetadze, B ;

Yamamoto, C ;

Okamoto, Y .

CHEMISTRY LETTERS, 2003, 32 (09) :850-851

[8]

Cho SI, 2002, ELECTROPHORESIS, V23, P972, DOI 10.1002/1522-2683(200203)23:6<972::AID-ELPS972>3.0.CO

[9]

2-F

[10]

de Boer T, 2000, ELECTROPHORESIS, V21, P3220, DOI 10.1002/1522-2683(20000901)21:15<3220::AID-ELPS3220>3.0.CO

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