Comparison of the digene hybrid capture system cytomegalovirus (CMV) DNA (Version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests using AcroMetrix OptiQuant CMV DNA quantification panels and specimens from allogeneic-stem-cell transplant recipients

被引:14
作者
Hanson, Kimberly E.
Reller, L. Barth
Kurtzberg, Joanne
Horwitz, Mitchell
Long, Gwynn
Alexander, Barbara D.
机构
[1] Duke Univ, Med Ctr, Div Infect Dis & Int Hlth, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Div Cellular Therapy, Durham, NC 27710 USA
[3] Duke Univ, Med Ctr, Dept Med, Pediat Blood & Marrow Transplant Program, Durham, NC 27710 USA
[4] Duke Univ, Med Ctr, Dept Pediat, Durham, NC 27710 USA
[5] Duke Univ, Med Ctr, Dept Pathol, Clin Microbiol Lab, Durham, NC 27710 USA
关键词
D O I
10.1128/JCM.02515-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Digene Hybrid Capture system cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN ReatArt CMV LightCycler PCR reagent tests were compared using whole-virus standards and plasma specimens collected from allogeneic-stem-cell transplant recipients. PCR assays showed better speed, sensitivity, and specificity.
引用
收藏
页码:1972 / 1973
页数:2
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