Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae

被引：393

作者：

Gerton, JL

DeRisi, J

Shroff, R

Lichten, M

Brown, PO

Petes, TD

机构：

[1] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Curriculum Genet & Mol Biol, Chapel Hill, NC 27599 USA

[3] Stanford Univ, Sch Med, Howard Hughes Med Inst, Dept Biochem, Stanford, CA 94305 USA

[4] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

[5] NCI, Biochem Lab, NIH, Bethesda, MD 20892 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 21期

关键词：

D O I：

10.1073/pnas.97.21.11383

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the yeast Saccharomyces cerevisiae, meiotic recombination is initiated by double-strand DNA breaks (DsBs). Meiotic DsBs occur at relatively high frequencies in some genomic regions (hotspots) and relatively low frequencies in others (coldspots). We used DNA microarrays to estimate variation in the level of nearby meiotic DSBs for all 6.200 yeast genes. Hotspots were nonrandomly associated with regions of high G + C base composition and certain transcriptional profiles. Coldspots were nonrandomly associated with the centromeres and telomeres.

引用

页码：11383 / 11390

页数：8

共 35 条

[31]

WU TC, 1995, GENETICS, V140, P55

[32] MEIOSIS-INDUCED DOUBLE-STRAND BREAK SITES DETERMINED BY YEAST CHROMATIN STRUCTURE [J].