M-type KCNQ2-KCNQ3 potassium channels are modulated by the KCNE2 subunit

被引：70

作者：

Tinel, N ^{[1
]}

Diochot, S ^{[1
]}

Lauritzen, I ^{[1
]}

Barhanin, J ^{[1
]}

Lazdunski, M ^{[1
]}

Borsotto, M ^{[1
]}

机构：

[1] CNRS, Inst Pharmacol Mol & Cellulaire, UPR 411, F-06560 Valbonne, France

来源：

FEBS LETTERS | 2000年 / 480卷 / 2-3期

关键词：

potassium channel; epilepsy; regulatory protein; immunoprecipitation; in situ hybridization;

D O I：

10.1016/S0014-5793(00)01918-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

KCNQ2 and KCNQ3 subunits belong to the six transmembrane domain K+ channel family and loss of function mutations are associated with benign familial neonatal convulsions. KCNE2 (MirP1) is a single transmembrane domain subunit first described to be a modulator of the HERG potassium channel in the heart. Here, we show that KCNE2 is present in brain, in areas which also express KCNQ2 and KCNQ3 channels, We demonstrate that KCNE2 associates with KCNQ2 and/or KCNQ3 subunits, In transiently transfected COS cells, KCNE2 expression produces an acceleration of deactivation kinetics of KCNQ2 and of the KCNQ2-KCNQ3 complex. Effects of two previously identified arrhythmogenic mutations of KCNE2 have also been analyzed. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：137 / 141

页数：5

共 22 条

[1] MiRP1 forms IKr potassium channels with HERG and is associated with cardiac arrhythmia [J].

Abbott, GW ;

Sesti, F ;

Splawski, I ;

Buck, ME ;

Lehmann, WH ;

Timothy, KW ;

Keating, MT ;

Goldstein, SAN .

CELL, 1999, 97 (02) :175-187

[2] Ion channels - A new wave for heart rhythms [J].

Attali, B .

NATURE, 1996, 384 (6604) :24-25

[3] IKs, a slow and intriguing cardiac K+ channel and its associated long QT diseases [J].

Barhanin, J ;

Attali, B ;

Lazdunski, L .

TRENDS IN CARDIOVASCULAR MEDICINE, 1998, 8 (05) :207-214

[4] K(v)LQT1 and IsK (minK) proteins associate to form the I-Ks cardiac potassium current [J].

Barhanin, J ;

Lesage, F ;

Guillemare, E ;

Fink, M ;

Lazdunski, M ;

Romey, G .

NATURE, 1996, 384 (6604) :78-80

[5] A potassium channel mutation in neonatal human epilepsy [J].

Biervert, C ;

Schroeder, BC ;

Kubisch, C ;

Berkovic, SF ;

Propping, P ;

Jentsch, TJ ;

Steinlein, OK .

SCIENCE, 1998, 279 (5349) :403-406

[6] AN AMINO-ACID MUTATION IN A POTASSIUM CHANNEL THAT PREVENTS INHIBITION BY PROTEIN-KINASE-C [J].

BUSCH, AE ;

VARNUM, MD ;

NORTH, RA ;

ADELMAN, JP .

SCIENCE, 1992, 255 (5052) :1705-1707

[7] A pore mutation in a novel KQT-like potassium channel gene in an idiopathic epilepsy family [J].

Charlier, C ;

Singh, NA ;

Ryan, SG ;

Lewis, TB ;

Reus, BE ;

Leach, RJ ;

Leppert, M .

NATURE GENETICS, 1998, 18 (01) :53-55

[8] Properties of KvLQT1 K+ channel mutations in Romano-Ward and Jervell and Lange-Nielsen inherited cardiac arrhythmias [J].

Chouabe, C ;

Neyroud, N ;

Guicheney, P ;

Lazdunski, M ;

Romey, G ;

Barhanin, J .

EMBO JOURNAL, 1997, 16 (17) :5472-5479

[9] KCNQ4, a novel potassium channel expressed in sensory outer hair cells, is mutated in dominant deafness [J].

Kubisch, C ;

Schroeder, BC ;

Friedrich, T ;

Lütjohann, B ;

El-Amraoui, A ;

Marlin, S ;

Petit, C ;

Jentsch, TJ .

CELL, 1999, 96 (03) :437-446

[10] Molecular cloning and functional expression of KCNQ5, a potassium channel subunit that may contribute to neuronal M-current diversity [J].

Lerche, C ;

Scherer, CR ;

Seebohm, G ;

Derst, C ;

Wei, AD ;

Busch, AE ;

Steinmeyer, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (29) :22395-22400

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