Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci

被引：240

作者：

Kariyama, R

Mitsuhata, R

Chow, JW

Clewell, DB

Kumon, H

机构：

[1] Okayama Univ, Sch Med, Dept Urol, Okayama 7008558, Japan

[2] Okayama Univ Hosp, Sect Infect Control, Okayama, Japan

[3] John D Dingell VA Med Ctr, Res & Med Serv, Detroit, MI USA

[4] Wayne State Univ, Sch Med, Div Infect Dis, Detroit, MI 48201 USA

[5] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Sch Med, Dept Immunol Microbiol, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 08期

关键词：

D O I：

10.1128/JCM.38.8.3092-3095.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

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收藏

页码：3092 / 3095

页数：4

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