Flow cytometry of mitotic cells

被引:31
作者
Anderson, HJ
de Jong, G
Vincent, I
Roberge, M
机构
[1] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V6T 1Z3, Canada
[2] British Columbia Canc Agcy, Div Med Oncol, Vancouver, BC V5Z 4E6, Canada
[3] Yeshiva Univ Albert Einstein Coll Med, Dept Pathol, New York, NY 10461 USA
关键词
cell cycle/mitosis; monoclonal antibody; flow cytometry; cell sorting;
D O I
10.1006/excr.1997.3862
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have developed a procedure using flow cytometric measurement of a mitosis-specific antigen that may be used to count mitotic cells and sort them from nonmitotic cells, The procedure may also be used in conjunction with measurement of cellular DNA content and of bromodeoxyuridine incorporation into cellular DNA to assign cells to the G1/G0, S, G2, or M phase of the cell cycle. (C) 1998 Academic Press.
引用
收藏
页码:498 / 502
页数:5
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