Cloning and nucleotide sequence of the DNA gyrase gyrA gene from Serratia marcescens and characterization of mutations in gyrA of quinolone-resistant clinical isolates

被引：23

作者：

Kim, JH

Cho, EH

Kim, KS

Kim, HY

Kim, YM ^{[1
]}

机构：

[1] Yonsei Univ, Dept Biol, Mol Microbiol Lab, Seoul 120749, South Korea

[2] Chosun Univ, Dept Sci Educ, Kwangju 501759, South Korea

[3] C&C Res Labs, Dept Pharmacol & Mol Biol, Kyunggi Do 445970, South Korea

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1998年 / 42卷 / 01期

关键词：

D O I：

10.1128/AAC.42.1.190

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined, An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens.

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页码：190 / 193

页数：4

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