Identification of a tRNA-specific nuclear export receptor

被引：312

作者：

Kutay, U

Lipowsky, G

Izaurralde, E

Bischoff, FR

Schwarzmaier, P

Hartmann, E

Gorlich, D ^{[1
]}

机构：

[1] Heidelberg Univ, Zentrum Mol Biol, D-69120 Heidelberg, Germany

[2] Univ Geneva, Dept Mol Biol, CH-1211 Geneva 4, Switzerland

[3] Deutsch Krebsforschungszentrum, Abt Mol Biol Mitose, D-69120 Heidelberg, Germany

[4] Max Delbruck Ctr Mol Med, D-13122 Berlin, Germany

来源：

MOLECULAR CELL | 1998年 / 1卷 / 03期

关键词：

D O I：

10.1016/S1097-2765(00)80036-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.

引用

页码：359 / 369

页数：11

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