Thermally reversible hydrogel modified capillary electrophoretic immunoassay of estradiol with laser-induced fluorescence detection

被引：24

作者：

Su, P ^{[1
]}

Wang, YC ^{[1
]}

Zhang, XX ^{[1
]}

Sun, L ^{[1
]}

Chang, WB ^{[1
]}

机构：

[1] Peking Univ, Dept Chem, Beijing 100871, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 418卷 / 02期

基金：

中国国家自然科学基金;

关键词：

hydrogel; capillary electrophoresis; laser-induced fluorescence; immunoassay;

D O I：

10.1016/S0003-2670(00)00959-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, thermally reversible hydrogel used as a replaceable packed material for capillary electrophoresis (CE) was examined. A simple and rapid method for detecting estradiol (E2) was developed, which used a strong affinity antibody (K-d=1.5 x 10(9)) as a selector for immunologically based separations in serum by CE, using an uncoated capillary. Monoclonal antibody (McAb) was linked to hydrogel and used in the separation of free fluorescein isothiocyanate (FITC) labeled antigen and free antigen with laser-induced fluorescence detector (LIF). Separating conditions were optimized. E2 could be determined, specifically by capillary electrophoretic immunoassay (CEIA), without the need for pre-incubation. The N-isopropylacrylamide (PNIPA) hydrogel, including antibody, was stable and could be stored for long periods at 4 degrees C. It can be used to determine E2 with good precision at sensitivities as low as 30 pg/ml. Details of the preparation of hydrogel cross-linked monoclonal antibody and of typical separations of bound and free antigen were described. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：137 / 143

页数：7

共 29 条

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