Cells overexpressing fructose-2,6-bisphosphatase showed enhanced pentose phosphate pathway flux and resistance to oxidative stress

被引：42

作者：

Boada, J

Roig, T

Perez, X

Gamez, A

Bartrons, R

Cascante, M

Bermúdez, J

机构：

[1] Univ Barcelona, Div Ciencies Salut, Dept Ciencies Fisiol 2, E-08907 Barcelona, Spain

[2] Univ Politecn Catalunya, EUETIB, Dept Engn Quim, Catalunya, Spain

[3] Univ Barcelona, Dept Bioquim & Biol Mol, Div Ciencies Expt, Barcelona, Spain

来源：

FEBS LETTERS | 2000年 / 480卷 / 2-3期

关键词：

glycolysis; 6-phosphofructo-2-kinase; fructose-2,6-bisphosphatase; fructose-2,6-bisphosphate; pentose phosphate pathway; microcalorimetry;

D O I：

10.1016/S0014-5793(00)01950-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in the content of fructose-2,6-bisphosphate, a modulator of glycolytic flux, also affect other metabolic fluxes such as the non-oxidative pentose phosphate pathway. Since this is the main source of precursors for biosynthesis in proliferating cells, PFK-2/FBPase-2 has been proposed as a potential target for neoplastic treatments, Here we provide evidence that cells with a low content of fructose-2,6-bisphosphate have a lower energy status than controls, but they are also less sensitive to oxidative stress. This feature is related to the activation of the oxidative branch of the pentose phosphate pathway and the increased production of NADPH. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：261 / 264

页数：4

共 27 条

[1]

[Anonymous], METHODS ENZYMOLOGY

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