Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells:: a technical update

被引：62

作者：

Kernéis, S

Caliot, E

Stubbe, H

Bogdanova, A

Kraehenbuhl, JP

Pringault, E ^{[1
]}

机构：

[1] Univ Lausanne, Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland

[2] Univ Lausanne, Inst Biochem, CH-1066 Epalinges, Switzerland

[3] Inst Pasteur, Dept Bacteriol & Mycol, Lab Interact Lymphoepitheliales, F-75015 Paris, France

来源：

MICROBES AND INFECTION | 2000年 / 2卷 / 09期

关键词：

coculture; M cells; Peyer's patches; gut; mucosal immunity;

D O I：

10.1016/S1286-4579(00)01266-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Peyer's patch lymphocytes cocultured with Caco-2 cells trigger the phenotypic conversion of enterocytes into cells that express morphological and functional hi-cell properties. We report a technical update for setting up this model, which will enable the study of M-cell biology, the identification by biochemical approaches of molecules involved in the interaction of microorganisms with hi cells, and the development of vectors that would efficiently target the mucosal immune system. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

引用

页码：1119 / 1124

页数：6

共 15 条

[11] M-CELL TRANSPORT OF VIBRIO-CHOLERAE FROM THE INTESTINAL LUMEN INTO PEYERS-PATCHES - A MECHANISM FOR ANTIGEN SAMPLING AND FOR MICROIAL TRANS-EPITHELIAL MIGRATION [J].