A microfluidic method for removal of the zona pellucida from mammalian embryos

被引：30

作者：

Zeringue, HC

Wheeler, MB

Beebe, DJ ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA

[2] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA

来源：

LAB ON A CHIP | 2005年 / 5卷 / 01期

关键词：

D O I：

10.1039/b406703g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Many in vitro procedures involve manipulation of the zona pellucida (chimerics, transgenics, biopsy). We have demonstrated a microfluidic channel network to precisely control ( spatially and temporally) the delivery of chemical treatments for the removal of the zona pellucida. Building devices in polydimethylsiloxane ( PDMS) with channel dimensions on the same order as that of the embryo diameter (similar to120 mum) allows precise control of the local fluid environment. The system uses pressure driven flows to control embryo positioning, embryo movement, and plug formation. Zona removal is achieved by briefly washing a plug of lysing agent ( acid Tyrode's medium) over the embryo.

引用

收藏

页码：108 / 110

页数：3

相关论文

共 17 条

[1] Monozygotic twinning following assisted conception: an analysis of 81 consecutive cases [J].

Alikani, M ;

Cekleniak, NA ;

Walters, E ;

Cohen, J .

HUMAN REPRODUCTION, 2003, 18 (09) :1937-1943

[2] Microfluidic technology for assisted reproduction [J].

Beebe, D ;

Wheeler, M ;

Zeringue, H ;

Walters, E ;

Raty, S .

THERIOGENOLOGY, 2002, 57 (01) :125-135

[3]

Duffy D., 1998, ANGEW CHEM INT EDIT, V37, P550

[4]

EDIOSAGIE E, 2003, COCHRANE DB SYST REV, V4, DOI DOI 10.1002/14651858

[5] Estimates of heterosis for in vitro embryo production using reciprocal crosses in cattle [J].

Fischer, AE ;

Bernal, DP ;

Gutierrez-Robayo, C ;

Rutledge, JJ .

THERIOGENOLOGY, 2000, 54 (09) :1433-1442

[6] Three-dimensional micro-channel fabrication in polydimethylsiloxane (PDMS) elastomer [J].

Jo, BH ;

Van Lerberghe, LM ;

Motsegood, KM ;

Beebe, DJ .

JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 2000, 9 (01) :76-81

[7] Comparison of the results of human embryo biopsy and outcome of PGD after zona drilling using acid Tyrode medium or a laser [J].

Joris, H ;

De Vos, A ;

Janssens, R ;

Devroey, P ;

Liebaers, I ;

Van Steirteghem, A .

HUMAN REPRODUCTION, 2003, 18 (09) :1896-1902

[8] Laser-assisted zona pellucida thinning prior to routine ICSI [J].

Moser, M ;

Ebner, T ;

Sommergruber, M ;

Gaisswinkler, U ;

Jesacher, K ;

Puchner, M ;

Wiesinger, R ;

Tews, G .

HUMAN REPRODUCTION, 2004, 19 (03) :573-578

[9] DERIVATION OF COMPLETELY CELL CULTURE-DERIVED MICE FROM EARLY-PASSAGE EMBRYONIC STEM-CELLS [J].

NAGY, A ;

ROSSANT, J ;

NAGY, R ;

ABRAMOWNEWERLY, W ;

RODER, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (18) :8424-8428

[10] PREGNANCIES AFTER INTRACYTOPLASMIC INJECTION OF SINGLE SPERMATOZOON INTO AN OOCYTE [J].

PALERMO, G ;

JORIS, H ;

DEVROEY, P ;

VANSTEIRTEGHEM, AC .

LANCET, 1992, 340 (8810) :17-18