A Nodule-Specific Protein Secretory Pathway Required for Nitrogen-Fixing Symbiosis

被引：200

作者：

Wang, Dong ^{[1
]}

Griffitts, Joel ^{[1
]}

Starker, Colby ^{[1
]}

Fedorova, Elena ^{[2
,3
]}

Limpens, Erik ^{[2
]}

Ivanov, Sergey ^{[2
,3
]}

Bisseling, Ton ^{[2
]}

Long, Sharon ^{[1
]}

机构：

[1] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[2] Wageningen Univ, Grad Sch Expt Plant Sci, Mol Biol Lab, NL-6708 PB Wageningen, Netherlands

[3] Russian Acad Sci, KA Timiryazev Plant Physiol Inst, Moscow 127392, Russia

来源：

SCIENCE | 2010年 / 327卷 / 5969期

基金：

美国国家卫生研究院;

关键词：

SIGNAL PEPTIDASE ACTIVITY; MEDICAGO-TRUNCATULA; ROOT-NODULES; ENDOPLASMIC-RETICULUM; GENE-EXPRESSION; MEMBRANE; FIXATION; MUTANTS; DEFINE; PLANT;

D O I：

10.1126/science.1184096

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1.

引用

收藏

页码：1126 / 1129

页数：4

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