Comparison of the T cell patterns in leprous and cutaneous sarcoid granulomas -: Presence of Vα24-invariant natural killer T cells in T-cell-reactive leprosy together with a highly biased T cell receptor Vα repertoire

被引:42
作者
Mempel, M
Flageul, B
Suarez, F
Ronet, C
Dubertret, L
Kourilsky, P
Gachelin, G
Musette, P
机构
[1] Inst Pasteur, Dept Immunol, Unite Biol Mol Gene, INSERM,U277, F-75015 Paris, France
[2] Hop St Louis, INSERM, U312, Paris, France
关键词
D O I
10.1016/S0002-9440(10)64562-2
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The T-cell-reactive (eg, tuberculoid and reversal) forms of leprosy represent a well-defined granulomatous reaction pattern against an invading pathogen. The immune response in cutaneous sarcoidosis is a granulomatous condition that pathologically Is very similar to T-cell reactive leprosy. However, it lacks a defined causative agent. In view of the role of NKT cells in murine granulomas induced by mycobacterial cell walls, we have searched for the presence of NKT cells in the cutaneous lesions of both leprosy and sarcoidosis. These cells were present in T-cell-reactive leprosy but were undetectable in cutaneous sarcoidosis, We have also studied the TCR V alpha repertoire in the two diseases. In addition to V alpha 24(+) NKT cells, all patients with T-cell-reactive leprosy showed a very restricted T-cell-reactive V alpha repertoire with a strong bias toward the use of the V alpha 6 and V alpha 14 segments. V alpha 6 and V alpha 14(+) T cells were polyclonal In terms of CDR3 length and J alpha usage. In contrast, most sarcoidosis patients showed a diverse usage of V alpha chains associated with clonal or oligoclonal expansions reminiscent of antigen-driven activation of conventional T cells. Thus the origin and perpetuation of the two kinds of granulomatous lesions appear to depend on altogether distinct T-cell recruiting mechanisms.
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收藏
页码:509 / 523
页数:15
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