FTIR spectroscopy studies of nucleic acid damage

被引：313

作者：

Dovbeshko, GI

Gridina, NY

Kruglova, EB

Pashchuk, OP

机构：

[1] Natl Acad Sci Ukraine, Inst Phys, UA-03039 Kiev, Ukraine

[2] Ukraine Acad Med Sci, Romodanov Inst Neurosurg, UA-04050 Kiev, Ukraine

[3] Natl Acad Sci Ukraine, Inst Radiophys & Elect, UA-61085 Kharkov, Ukraine

来源：

TALANTA | 2000年 / 53卷 / 01期

关键词：

RNA; DNA; FTIR spectra; structural damage; tumour cells; low-dose gamma-irradiation;

D O I：

10.1016/S0039-9140(00)00462-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fourier transform infrared (FTIR) spectra of RNA isolated from tumour brain (glioma) and DNA isolated from low-dose gamma-irradiated epididymis cells of rats from the Chernobyl accident zone have been investigated in the 700-4000 cm(-1) region. The total absorbed radiation doses were equal to or less than 57 cGy. Observed changes in the FTIR spectra represent the damage in the primary, secondary and tertiary structure of nucleic acid, which seem to be connected with modification of bases and sugars, and redistribution of the H-bond network. FTIR data for tumour and gamma-irradiated nucleic acid show some similar features. A great amount of statistical data and good mathematical approaches are needed for the use of these data as diagnostic criteria. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：233 / 246

页数：14

共 21 条

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