Differential Downregulation of ACE2 by the Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus and Human Coronavirus NL63

被引：376

作者：

Glowacka, Ilona ^{[1
]}

Bertram, Stephanie ^{[1
]}

Herzog, Petra ^{[2
,3
]}

Pfefferle, Susanne ^{[2
]}

Steffen, Imke ^{[1
]}

Muench, Marcus O. ^{[4
,5
]}

Simmons, Graham ^{[4
,5
]}

Hofmann, Heike ^{[6
]}

Kuri, Thomas ^{[7
]}

Weber, Friedemann ^{[7
]}

Eichler, Jutta ^{[8
]}

Drosten, Christian ^{[9
]}

Poehlmann, Stefan ^{[1
]}

机构：

[1] Hannover Med Sch, Inst Virol, D-30625 Hannover, Germany

[2] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany

[3] Qiagen Hamburg GmbH, D-22767 Hamburg, Germany

[4] Univ Calif San Francisco, Blood Syst Res Inst, San Francisco, CA 94143 USA

[5] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA

[6] Univ Kiel, Dept Med Microbiol & Virol, D-24105 Kiel, Germany

[7] Univ Freiburg, Dept Virol, D-79008 Freiburg, Germany

[8] Univ Erlangen Nurnberg, Dept Med Chem, D-91052 Erlangen, Germany

[9] Univ Bonn, Med Ctr, Inst Virol, D-53127 Bonn, Germany

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 02期

关键词：

ANGIOTENSIN-CONVERTING ENZYME-2; SARS-COV; FUNCTIONAL RECEPTOR; TISSUE DISTRIBUTION; INFECTION; ANGIOTENSIN-CONVERTING-ENZYME-2; CHILDREN; PATHOGENESIS; EXPRESSION; DISEASE;

D O I：

10.1128/JVI.01248-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The human coronaviruses (CoVs) severe acute respiratory syndrome (SARS)-CoV and NL63 employ angiotensin-converting enzyme 2 (ACE2) for cell entry. It was shown that recombinant SARS-CoV spike protein (SARS-S) downregulates ACE2 expression and thereby promotes lung injury. Whether NL63-S exerts a similar activity is yet unknown. We found that recombinant SARS-S bound to ACE2 and induced ACE2 shedding with higher efficiency than NL63-S. Shedding most likely accounted for the previously observed ACE2 down-regulation but was dispensable for viral replication. Finally, SARS-CoV but not NL63 replicated efficiently in ACE2-positive Vero cells and reduced ACE2 expression, indicating robust receptor interference in the context of SARS-CoV but not NL63 infection.

引用

页码：1198 / 1205

页数：8

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