High-molecular-weight tropomyosins localize to the contractile rings of dividing CNS cells but are absent from malignant pediatric and adult CNS tumors

被引:52
作者
Hughes, JAI
Cooke-Yarborough, CM
Chadwick, NC
Schevzov, G
Arbuckle, SM
Gunning, P
Weinberger, RP
机构
[1] Childrens Hosp Westmead, Oncol Res Unit, Westmead, NSW, Australia
[2] Childrens Hosp Westmead, Dept Pathol, Westmead, NSW, Australia
[3] Univ Sydney, Dept Paediat & Child Hlth, Sydney, NSW 2006, Australia
关键词
cytoskeleton; astrocyte; cancer; cell division; cytokinesis;
D O I
10.1002/glia.10174
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Tropomyosin has been implicated in the control of actin filament dynamics during cell migration, morphogenesis, and cytokinesis. In order to gain insight into the role of tropomyosins in cell division, we examined their expression in developing and neoplastic brain tissue. We found that the high-molecular-weight tropomyosins are downregulated at birth, which correlates with glial cell differentiation and withdrawal of most cells from the cell cycle. Expression of these isoforms was restricted to proliferative areas in the embryonic brain and was absent from the adult, where the majority of cells are quiescent. However, they were induced under conditions where glial cells became proliferative in response to injury. During cytokinesis, these tropomyosin isoforms were associated with the contractile ring. We also investigated tropomyosin expression in neoplastic CNS tissues. Low-grade astrocytic tumors expressed high-molecular-weight tropomyosins, while highly malignant CNS tumors of diverse origin did not (P less than or equal to 0.001). Furthermore, high-molecular-weight tropomyosins were absent from the contractile ring in highly malignant astrocytoma cells. Our findings suggest a role for high-molecular-weight tropomyosins in astrocyte cytokinesis, although highly malignant CNS tumors are still able to undergo cell division in their absence. Additionally, the correlation between high-molecular-weight tropomyosin expression and tumor grade suggests that tropomyosins are potentially useful as indicators of CNS tumor grade. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:25 / 35
页数:11
相关论文
共 59 条
[51]  
Temm-Grove CJ, 1998, CELL MOTIL CYTOSKEL, V40, P393, DOI 10.1002/(SICI)1097-0169(1998)40:4<393::AID-CM7>3.0.CO
[52]  
2-C
[53]  
Wang FL, 1996, CANCER RES, V56, P3634
[54]   FORCED EXPRESSION OF CHIMERIC HUMAN FIBROBLAST TROPOMYOSIN MUTANTS AFFECTS CYTOKINESIS [J].
WARREN, KS ;
LIN, JLC ;
MCDERMOTT, JP ;
LIN, JJC .
JOURNAL OF CELL BIOLOGY, 1995, 129 (03) :697-708
[55]  
Weinberger R, 1996, J NEUROSCI, V16, P238
[56]   INDUCTION OF NEURON-SPECIFIC TROPOMYOSIN MESSENGER-RNAS BY NERVE GROWTH-FACTOR IS DEPENDENT ON MORPHOLOGICAL-DIFFERENTIATION [J].
WEINBERGER, RP ;
HENKE, RC ;
TOLHURST, O ;
JEFFREY, PL ;
GUNNING, P .
JOURNAL OF CELL BIOLOGY, 1993, 120 (01) :205-215
[57]   A METHOD FOR THE QUANTITATIVE RECOVERY OF PROTEIN IN DILUTE-SOLUTION IN THE PRESENCE OF DETERGENTS AND LIPIDS [J].
WESSEL, D ;
FLUGGE, UI .
ANALYTICAL BIOCHEMISTRY, 1984, 138 (01) :141-143
[58]  
Wong K, 2000, CELL MOTIL CYTOSKEL, V45, P121, DOI 10.1002/(SICI)1097-0169(200002)45:2<121::AID-CM4>3.0.CO
[59]  
2-#