Microenvironmental influences on human B-cell development

被引：25

作者：

Bertrand, FE ^{[1
]}

Eckfeldt, CE ^{[1
]}

Fink, JR ^{[1
]}

Lysholm, AS ^{[1
]}

Pribyl, JAR ^{[1
]}

Shah, N ^{[1
]}

LeBien, TW ^{[1
]}

机构：

[1] Univ Minnesota, Ctr Canc, Minneapolis, MN 55455 USA

来源：

IMMUNOLOGICAL REVIEWS | 2000年 / 175卷

关键词：

D O I：

10.1034/j.1600-065X.2000.017513.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mammalian B-cell development can be viewed as a developmental performance with several acts. The acts are represented by check-points centered around commitment to the B-lineage and functional Ig gene rearrangement - culminating in expression of the pre-B-cell receptor (pre-BCR) and the BCR. Progression of cells through these checkpoints is profoundly influenced by the fetal liver and adult bone marrow (BM) stromal cell microenvironments. Our laboratory has developed a model of human B-cell development that utilizes freshly isolated/non-transformed human BM stromal cells as an in vitro microenvironment. Human CD34(+) hematopoietic stem cells plated in this human BM stromal cell microenvironment commit to the B lineage and progress through the pre-BCR and BCR checkpoints. This human BM stromal cell microenvironment also provides survival signals that prevent apoptosis in human B-lineage cells. Human B-lineage cells exhibit differential expression of Notch receptors and human BM stromal cells express the Notch ligand Jagged-1. These results suggest a potential role for Notch in regulating B-lineage commitment and/or progression through the pre-BCR and BCR checkpoints.

引用

页码：175 / 186

页数：12

共 72 条

[31] Inhibition of granulocytic differentiation by mNotch1 [J].