Environmental regulation of the anaerobic oxidation of methane:: a comparison of ANME-I and ANME-II communities

被引:229
作者
Nauhaus, K
Treude, T
Boetius, A
Krüger, M
机构
[1] Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany
[2] Alfred Wegener Inst Polar & Marine Res, D-27515 Bremerhaven, Germany
[3] Int Jacobs Univ Bremen, D-28759 Bremen, Germany
关键词
D O I
10.1111/j.1462-2920.2004.00669.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The anaerobic oxidation of methane (AOM) is one of the major sinks for methane on earth and is known to be mediated by at least two phylogenetically different groups of anaerobic methanotrophic Archaea (ANME-I and ANME-II). We present the first comparative in vitro study of the environmental regulation and physiology of these two methane-oxidizing communities, which occur naturally enriched in the anoxic Black Sea (ANME-I) and at Hydrate Ridge (ANME-II). Both types of methanotrophic communities are associated with sulfate-reducing-bacteria (SRB) and oxidize methane anaerobically in a 1:1 ratio to sulfate reduction (SR). They responded sensitively to elevated methane partial pressures with increased substrate turnover. The ANME-II-dominated community showed significantly higher cell-specific AOM rates. Besides sulfate, no other electron acceptor was used for AOM. The processes of AOM and SR could not be uncoupled by feeding the SRB with electron donors such as acetate, formate or molecular hydrogen. AOM was completely inhibited by the addition of bromoethanesulfonate in both communities, indicating the participation of methanogenic enzymes in the process. Temperature influenced the intensity of AOM, with ANME-II being more adapted to cold temperatures than ANME-I. The variation of other environmental parameters, such as sulfate concentration, pH and salinity, did not influence the activity of both communities. In conclusion, the ecological niches of methanotrophic Archaea seem to be mainly defined by the availability of methane and sulfate, but it remains open which additional factors lead to the dominance of ANME-I or -II in the environment.
引用
收藏
页码:98 / 106
页数:9
相关论文
共 43 条
[1]   Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Gol [J].
Abken, HJ ;
Tietze, M ;
Brodersen, J ;
Bäumer, S ;
Beifuss, U ;
Deppenmeier, U .
JOURNAL OF BACTERIOLOGY, 1998, 180 (08) :2027-2032
[2]   ANAEROBIC OXIDATION OF SATURATED-HYDROCARBONS TO CO2 BY A NEW TYPE OF SULFATE-REDUCING BACTERIUM [J].
AECKERSBERG, F ;
BAK, F ;
WIDDEL, F .
ARCHIVES OF MICROBIOLOGY, 1991, 156 (01) :5-14
[3]   INHIBITION EXPERIMENTS ON ANAEROBIC METHANE OXIDATION [J].
ALPERIN, MJ ;
REEBURGH, WS .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 50 (04) :940-945
[4]  
ANGELIS MA, 1991, LIMNOL OCEANOGR, V36, P565
[5]   A marine microbial consortium apparently mediating anaerobic oxidation of methane [J].
Boetius, A ;
Ravenschlag, K ;
Schubert, CJ ;
Rickert, D ;
Widdel, F ;
Gieseke, A ;
Amann, R ;
Jorgensen, BB ;
Witte, U ;
Pfannkuche, O .
NATURE, 2000, 407 (6804) :623-626
[6]   Hydrate Ridge: a natural laboratory for the study of microbial life fueled by methane from near-surface gas hydrates [J].
Boetius, A ;
Suess, E .
CHEMICAL GEOLOGY, 2004, 205 (3-4) :291-310
[7]   DIFFUSION OF THE INTERSPECIES ELECTRON CARRIERS H-2 AND FORMATE IN METHANOGENIC ECOSYSTEMS AND ITS IMPLICATIONS IN THE MEASUREMENT OF KM FOR H-2 OR FORMATE UPTAKE [J].
BOONE, DR ;
JOHNSON, RL ;
LIU, Y .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (07) :1735-1741
[9]   GLOBAL BUDGETS FOR NON-CO2 GREENHOUSE GASES [J].
CRUTZEN, PJ .
ENVIRONMENTAL MONITORING AND ASSESSMENT, 1994, 31 (1-2) :1-15
[10]  
EHALT DH, 1974, TELLUS, V26, P58