The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia

被引：14

作者：

Secondo, Agnese ^{[1
]}

Staiano, Ilaria Rosaria ^{[1
]}

Scorziello, Antonella ^{[1
]}

Sirabella, Rossana ^{[1
]}

Boscia, Francesca ^{[1
]}

Adornetto, Annagrazia ^{[1
]}

Canzoniero, Lorella Maria Teresa ^{[1
]}

Di Renzo, Gianfranco ^{[1
]}

Annunziato, Lucio ^{[1
]}

机构：

[1] Univ Naples Federico II, Sch Med, Dept Neurosci, Div Pharmacol, I-80131 Naples, Italy

来源：

SODIUM-CALCIUM EXCHANGE AND THE PLASMA MEMBRANE CA2+-ATPASE IN CELL FUNCTION: FIFTH INTERNATIONAL CONFERENCE | 2007年 / 1099卷

关键词：

Na+/Ca2+ exchanger; chemical hypoxia; neuroprotection; Ca2+](i) homeostasis;

D O I：

10.1196/annals.1387.052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+](i) increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.

引用

页码：481 / 485

页数：5

共 21 条

[1] Pharmacological evidence that the activation of the Na+-Ca2+ exchanger protects C6 glioma cells during chemical hypoxia [J].