O-(acridinium)hydroxylamine (AHA):: a reagent for the preparation of chemiluminescent acridinium oxime (AO)-steroid conjugates

被引:6
作者
Adamczyk, M [1 ]
Grote, J [1 ]
Mattingly, PG [1 ]
Pan, Y [1 ]
机构
[1] Abbott Labs, Dept Chem, Div Diagnost, Dept 9NM, Abbott Pk, IL 60064 USA
关键词
O-(acridinium)hydroxylamine; acridinium steroid conjugates; tracers;
D O I
10.1016/S0039-128X(00)00095-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
O-(Acridinium)hydroxylamine (AHA) reacted with a representative sample of ore-steroids (6-oxoestradiol, estrone, norethindrone, cortisol, progesterone, digoxin dialdehyde, and digitoxin dialdehyde) to produce chemiluminescent acridinium oxime (AO) conjugates in a single step in 37-68% yield after preparative HPLC. (C) 2000 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:387 / 394
页数:8
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