A Facile Method To Encapsulate Proteins in Silica Nanoparticles: Encapsulated Green Fluorescent Protein as a Robust Fluorescence Probe

被引：82

作者：

Cao, Aoneng ^{[1
]}

Ye, Zhangmei ^{[1
]}

Cai, Zhengwei ^{[1
]}

Dong, Erya ^{[1
]}

Yang, Xiaowei ^{[1
]}

Liu, Gangbo ^{[1
]}

Deng, Xiaoyong ^{[1
]}

Wang, Yanli ^{[1
]}

Yang, Sheng-Tao ^{[2
]}

Wang, Haifang ^{[1
]}

Wu, Minghong ^{[1
]}

Liu, Yuanfang ^{[1
,2
]}

机构：

[1] Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China

[2] Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 17期

关键词：

fluorescent probes; immobilization; nanoparticles; polyhistidine tag; proteins; QUANTUM DOTS; IN-VIVO; PROTEOMICS;

D O I：

10.1002/anie.200906883

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

(Figure Presented) Si'l vous plait? A facile and general method has been developed to encapsulate polyhistidine-tagged proteins in silica nanoparticles (NPs; gray, see picture) using calcium ions (yellow). The enhanced green fluorescence protein (EGFP) encapsulated in the silica NPs shows a substantial increase in fluorescence intensity and stability against denaturants, protease, and heat. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3022 / 3025

页数：4

共 24 条

[1]

Bioinspired enzyme encapsulation for biocatalysis [J].