Interfamily transfer of a plant pattern-recognition receptor confers broad-spectrum bacterial resistance

被引：379

作者：

Lacombe, Severine ^{[1
]}

Rougon-Cardoso, Alejandra ^{[1
]}

Sherwood, Emma ^{[1
]}

Peeters, Nemo ^{[2
]}

Dahlbeck, Douglas ^{[4
]}

van Esse, H. Peter ^{[3
]}

Smoker, Matthew ^{[1
]}

Rallapalli, Ghanasyam ^{[1
]}

Thomma, Bart P. H. J. ^{[3
]}

Staskawicz, Brian ^{[4
]}

Jones, Jonathan D. G. ^{[1
]}

Zipfel, Cyril ^{[1
]}

机构：

[1] Sainsbury Lab, Norwich, Norfolk, England

[2] INRA, CNRS, Unite Mixte Rech, Lab Interact Plantes Microorganismes, Castanet Tolosan, France

[3] Wageningen Univ, Phytopathol Lab, Wageningen, Netherlands

[4] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

来源：

NATURE BIOTECHNOLOGY | 2010年 / 28卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

PSEUDOMONAS-SYRINGAE; INNATE IMMUNITY; DISEASE RESISTANCE; TRANSGENIC PLANTS; ARABIDOPSIS; PERCEPTION; TOMATO; GENE; EFFECTORS; DEFENSE;

D O I：

10.1038/nbt.1613

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant diseases cause massive losses in agriculture. Increasing the natural defenses of plants may reduce the impact of phytopathogens on agricultural productivity. Pattern-recognition receptors (PRRs) detect microbes by recognizing conserved pathogen-associated molecular patterns (PAMPs)(1-3). Although the overall importance of PAMP-triggered immunity for plant defense is established(2,3), it has not been used to confer disease resistance in crops. We report that activity of a PRR is retained after its transfer between two plant families. Expression of EFR (ref. 4), a PRR from the cruciferous plant Arabidopsis thaliana, confers responsiveness to bacterial elongation factor Tu in the solanaceous plants Nicotiana benthamiana and tomato (Solanum lycopersicum), making them more resistant to a range of phytopathogenic bacteria from different genera. Our results in controlled laboratory conditions suggest that heterologous expression of PAMP recognition systems could be used to engineer broad-spectrum disease resistance to important bacterial pathogens, potentially enabling more durable and sustainable resistance in the field.

引用

页码：365 / U94

页数：6

共 33 条

[1]

[Anonymous], 2009, 1109 ROYAL SOC

[2] A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition Receptors [J].