Molecular epidemiology of nga and NAD glycohydrolase/ADP-ribosyltransferase activity among Streptococcus pyogenes causing streptococcal toxic shock syndrome

被引:55
作者
Stevens, DL
Salmi, DB
McIndoo, ER
Bryant, AE
机构
[1] Vet Affairs Med Ctr, Dis Sect, Boise, ID 83702 USA
[2] Univ Idaho, Moscow, ID 83843 USA
[3] Univ Washington, Sch Med, Dept Med, Seattle, WA USA
关键词
D O I
10.1086/315850
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Severe invasive group A streptococcal (GAS) infections emerged in the late 1980s, yet no single virulence factor has been common to all isolates from infected patients, A strong association was recently found between isolates of such cases (regardless of M type) acid the production of NAD glycohydrolase (NADase), Of interest, all M-1 strains isolated after 1988 were positive for NADase, whereas virtually all M-1 GAS were previously negative for NADase. Genetic analysis demonstrated Chat GAS isolates were >96% identical in nga and >99% identical in their upstream regulatory sequences. Furthermore, because NADase-negative strains did not produce immunoreactive NADase, we concluded that additional regulatory element(s) control NADase production, NADase purified from GAS altered neutrophil-directed migration and chemiluminescence responses and had potent ADP-ribosyltransferase activity In summary, the temporal relationship of NADase expression, alone or with other streptococcal virulence factors, may contribute to the pathogenesis of invasive GAS infections.
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页码:1117 / 1128
页数:12
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