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pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferase

被引：17
作者：
Fraga, H
da Silva, JCGE
Fontes, R
机构：
[1] Univ Porto, Fac Ciencias, Dept Chem, LAQUIPAI, P-4169007 Oporto, Portugal
[2] Univ Porto, Fac Med, Biochem Dept U38 FCT, P-4200319 Oporto, Portugal
来源：
FEBS LETTERS | 2003年 / 543卷 / 1-3期
关键词：
adenosine(5 ')tetraphospho(5 ')adenosine; dehydroluciferin; dinucleoside polyphosphate; firefly luciferase; luciferin;
D O I：
10.1016/S0014-5793(03)00382-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Previous results have shown that an oxidizing product of firefly luciferin, dehydroluciferyl-adenylate, is the main intermediate in the process of synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase (EC 1.13.12.7). However, we have found that the pH effects on the luciferase oxidizing processes and on the synthesis of dinucleoside polyphosphate are opposite: acidic assay media enhance the synthesis of dinucleoside polyphosphate and inhibit the oxidizing processes. The reason for this apparent contradiction lies on the activation effect of low pH on the adenylate transfer reaction from dehydroluciferyl-adenylate to the acceptor nucleotide. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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页码：37 / 41
页数：5
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