Adsorption dynamics of L-glutamic acid copolymers at a heptane/water interface

被引:36
作者
Beverung, CJ [1 ]
Radke, CJ [1 ]
Blanch, HW [1 ]
机构
[1] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA
关键词
protein adsorption; liquid/liquid interface; interfacial tension; polyamino acids;
D O I
10.1016/S0301-4622(97)00117-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Random copolymers of glutamic acid (glu-ala, glu-leu, glu-phe, glu-tyr) were employed to investigate the relationship between side chain structure and peptide charge on adsorption behavior at an oil/water boundary. Adsorption of a series of glutamate copolymers at a heptane/water interface was examined by the dynamic pendant-drop method to determine interfacial tension. Incorporation of leucine or phenylalanine into a glutamate copolymer results in greater tension reduction than incorporation of alanine or tyrosine. These effects are amplified at pH values near the isoelectric point of glutamate, where macroscopic adsorbed films of glu-leu and glu-phe exhibit gel-like properties in response to interfacial area compression. Differences in interfacial tension behavior of glu-tyr and glu-phe indicate the importance of the tyrosine p-hydroxyl group on adsorption and aggregation at the oil/water interface. (C) 1998 Elsevier Science B.V.
引用
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页码:121 / 132
页数:12
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