Angiogenin-induced tRNA-derived Stress-induced RNAs Promote Stress-induced Stress Granule Assembly

被引：394

作者：

Emara, Mohamed M. ^{[3
]}

Ivanov, Pavel

Hickman, Tyler

Dawra, Nemisha

Tisdale, Sarah

Kedersha, Nancy

Hu, Guo-Fu ^{[2
]}

Anderson, Paul ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Brigham & Womens Hosp, Div Rheumatol Immunol & Allergy, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[3] Cairo Univ, Sch Vet Med, Dept Virol, Giza 12211, Egypt

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 14期

基金：

美国国家卫生研究院;

关键词：

EUKARYOTIC TRANSLATION INITIATION; INHIBITS TRANSLATION; SURVIVAL; PHOSPHORYLATION; EXPRESSION; TRANSCRIPTION; RECRUITMENT; APOPTOSIS; PROTEINS; PATHWAY;

D O I：

10.1074/jbc.M109.077560

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Angiogenin (ANG) is a secreted ribonuclease that cleaves tRNA to initiate a stress-response program in mammalian cells. Here we show that ANG inhibits protein synthesis and promotes arsenite-and pateamine A-induced assembly of stress granules (SGs). These effects are abrogated in cells transfected with the ANG inhibitor RNH1. Transfection of natural or synthetic 5'-but not 3'-tRNA fragments (tRNA-derived stress-induced RNAs; tiRNAs) induces the phospho-eukaryotic translation initiation factor 2 alpha-independent assembly of SGs. Natural 5'-tiRNAs but not 3'-tiRNAs are capped with a 5'-monophosphate that is required for optimal SG assembly. These findings reveal that SG assembly is a component of the ANG- and tiRNA-induced stress response program.

引用

页码：10959 / 10968

页数：10

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