The Hop/Sti1-Hsp90 Chaperone Complex Facilitates the Maturation and Transport of a PAMP Receptor in Rice Innate Immunity

被引：168

作者：

Chen, Letian ^{[1
]}

Hamada, Satoshi ^{[1
]}

Fujiwara, Masayuki ^{[1
]}

Zhu, Tingheng ^{[1
]}

Thao, Nguyen Phuong ^{[1
]}

Wong, Hann Ling ^{[1
]}

Krishna, Priti ^{[2
]}

Ueda, Takashi ^{[3
]}

Kaku, Hanae ^{[4
]}

Shibuya, Naoto ^{[4
]}

Kawasaki, Tsutomu ^{[1
]}

Shimamoto, Ko ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Plant Mol Genet Lab, Ikoma 6300192, Japan

[2] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada

[3] Univ Tokyo, Dept Biol Sci, Grad Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

[4] Meiji Univ, Dept Life Sci, Fac Agr, Tama Ku, Kanagawa 2148571, Japan

来源：

CELL HOST & MICROBE | 2010年 / 7卷 / 03期

基金：

日本学术振兴会;

关键词：

SMALL GTPASE RAC; DEFECTIVE BRASSINOSTEROID RECEPTOR; HETEROTRIMERIC G-PROTEIN; STRESS-INDUCIBLE GENE; DISEASE RESISTANCE; CELL-DEATH; ENDOPLASMIC-RETICULUM; SECRETORY PATHWAY; PLASMA-MEMBRANE; DOWN-REGULATION;

D O I：

10.1016/j.chom.2010.02.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs) represents a critical first step of innate defense in plants and animals. However, maturation and transport of PRRs are not well understood. We find that the rice chitin receptor OsCERK1 interacts with Hsp90 and its cochaperone Hop/Sti1 in the endoplasmic reticulum (ER). Hop/Sti1 and Hsp90 are required for efficient transport of OsCERK1 from the ER to the plasma membrane (PM) via a pathway dependent on Sari, a small GTPase which regulates ER-to-Golgi trafficking. Further, Hop/Sti1 and Hsp90 are present at the PM in a complex (designated the "defensome") with OsRac1, a plant-specific Rho-type GTPase. Finally, Hop/Sti1 was required for chitin-triggered immunity and resistance to rice blast fungus. Our results suggest that the Hop/Sti1-Hsp90 chaperone complex plays an important and likely conserved role in the maturation and transport of PRRs and may function to link PRRs and Rac/Rop GTPases.

引用

页码：185 / 196

页数：12

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