Investigation of tomato plant defence response to tobacco mosaic virus by determination of methyl salicylate with SPME-capillary GC-MS

被引：26

作者：

Deng, CH

Zhang, XM ^{[1
]}

Zhu, WM

Qian, J

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Acad Agr Sci, Shanghai 201106, Peoples R China

[3] Fudan Univ, Coll Life Sci, Shanghai 200433, Peoples R China

来源：

CHROMATOGRAPHIA | 2004年 / 59卷 / 3-4期

关键词：

gas ckromatograpky-mass spectrometry; headspace SPME; airborne signalling molecule; methyl salicylate from tomatoes; tobacco mosaic virus;

D O I：

10.1365/s10337-003-0144-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Headspace SPME was applied to investigation of tomato plant defence response to tobacco mosaic virus (TMV) by determination of compounds emitted from tomato plants at the extraction conditions of 25 degreesC and 15 min. It was found that TMV-inoculated tomato plant released large amount of methyl salicylate (MeSA) as response to TMV and MeSA concentration changed dramatically with time after inoculation. Gaseous MeSA as a signaling compound could induce in surrounding tomato plants to produce salicylic acid (SA) and synthesize and release MeSA. These results show that MeSA might be an airborne plant-signalling molecule in tomato plant response to TMV. The present method provided low detection limit of 2.0 ng L(-1) and needed little sample preparation time (15 min), so the method makes it easy to find the critical times of tomato plant response to TMV by fast determination of MeSA released from tomato plant.

引用

页码：263 / 268

页数：6

共 26 条

[1] SOLID-PHASE MICROEXTRACTION WITH THERMAL-DESORPTION USING FUSED-SILICA OPTICAL FIBERS [J].