Mass spectrometric detection and measurement of N2-(2′-deoxyguanosin-8-yl)PhIP adducts in DNA

被引：20

作者：

Crosbie, SJ ^{[1
]}

Murray, S ^{[1
]}

Boobis, AR ^{[1
]}

Gooderham, NJ ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Div Med, Sect Clin Pharmacol, London W12 0NN, England

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 744卷 / 01期

关键词：

DNA; 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine;

D O I：

10.1016/S0378-4347(00)00227-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary column gas chromatography-electron-capture mass spectrometry (GC-MS) and microbore liquid chromatography-positive ion electrospray mass spectrometry (LC-MS) have been used to measure the carcinogenic, food-derived, heterocyclic amine 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) adducted at C-8 of deoxyguanosine in DNA. For CC-MS analysis, PhIP was released from adducted DNA by alkaline hydrolysis and analysed as the di(3,5-bistrifluoromethylbenzyl) derivative, while for LC-MS analysis, the nucleoside N-2-(2'-deoxyguanosin-8-yl)PhIP was generated by enzymic digestion of DNA and analysed intact. A deuterated analogue of N-2-(2'-deoxyguanosin-8-yl)PhIP was used as an internal standard in both assays, which each had a limit of quantification of 200 pg/500 mu g DNA. The two methods were used to analyse DNA extracted from h1A2v2 cells and HCT116 cells that had been exposed to PhIP. (C) 2000 Elsevier Science BN. All rights reserved.

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页码：55 / 64

页数：10

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