Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata

被引：33

作者：

Funkenstein, B

Bowman, CJ

Denslow, ND

Cardinali, M

Carnevali, O

机构：

[1] Natl Inst Oceanog, Oceanog & Limnol Res, IL-31080 Haifa, Israel

[2] Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA

[3] Univ Ancona, Dept Biol, Ancona, Italy

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2000年 / 167卷 / 1-2期

关键词：

fish; liver; vitellogenin cDNA and protein; transthyretin; ELISA; RNA blot hybridization;

D O I：

10.1016/S0303-7207(00)00301-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of Sparus aurata male treated with 17 beta-estradiol (E-2). E-2 treatment of S. aurata males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E-2 treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

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页码：33 / 41

页数：9

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