A supercoiled DNA-modified mercury electrode-based biosensor for the detection of DNA strand cleaving agents

被引：51

作者：

Fojta, M

Stankova, V

Palecek, E

Koscielniak, P

Mitas, J

机构：

[1] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic

[2] DIAMO State Enterprise, Div GEAM, Dolni Rozinka 59251, Czech Republic

来源：

TALANTA | 1998年 / 46卷 / 01期

关键词：

supercoiled DNA; DNA-modified electrode; electrochemical biosensor; center dot OH radicals; DNA damage; environmental analysis;

D O I：

10.1016/S0039-9140(97)00281-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA-damaging agents in the environment represent a serious danger to human health. We use a supercoiled DNA-modified mercury electrode as a fast-response biosensor for the detection of DNA strand cleaving agents. The sensor is based on a strong difference between the a.c. voltammetric responses of covalently closed circular (supercoiled) and of open circular (nicked) plasmid DNA. We show that the sensor can detect hydroxyl radicals in laboratory-prepared solutions and in various natural and industrial water samples. The sensor is also capable of detecting unknown DNA-damaging agents in industrial waters. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：155 / 161

页数：7

共 26 条

[11] Site-specific DNA damage induced by NADH in the presence of copper(II): Role of active oxygen species [J].