Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone

被引：31

作者：

Kawanishi, M

Enya, T

Suzuki, H

Takebe, H

Matsui, S

Yagi, T

机构：

[1] Kyoto Univ, Fac Med, Dept Radiat Genet, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Res Ctr Environm Qual Control, Otsu, Shiga 5200811, Japan

[3] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto 6068502, Japan

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2000年 / 470卷 / 02期

基金：

日本学术振兴会;

关键词：

3-nitrobenznathrone; DAN adduct; P-32-postlabelling analysis; human cell;

D O I：

10.1016/S1383-5742(00)00053-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

3-Nitrobenzanthrone (NBA) is one of the most mutagenic nitroaromatic compounds that has been found recently in diesel exhaust and airborne particles. A [P-32]-postlabelling analysis was carried out to examine the adducts in DNA from human hepatoma HepG2 cells treated with NEA. Two major and two minor adduct spots were obtained in the analysis. The structure of the compound obtained from one of the minor adduct spots was identified to be N-acetyl-3-amino-2-(2'-deoxyguanosin-3',5'-bisphosphate-8-yl)-benzanthrone, based on identical mobility of the compound with that of synthetic standards in thin-layer chromatography and high performance liquid chromatography. This substance is the identical adduct found in our previous in vitro study. The yet-unidentified major adduct spots may be guanosin- and adenosin-benzanthrone adducts without the N-acetyl group. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：133 / 139

页数：7

共 13 条

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